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Asymbiotic culture of Cattleya intermedia Graham (Orchidaceae): the influence of macronutrient salts and sucrose concentrations on survival and development of plantlets Acta Botanica
Sasamori,Márcio Hisayuki; Endres Júnior,Delio; Droste,Annette.
ABSTRACTCattleya intermediais an Atlantic Forest species endemic to Brazil that is classed as vulnerable on the list of threatened species. In this study, C. intermedia plantlets were micropropagated in an asymbiotic culture and the influence of different concentrations of sucrose (15, 30, 45 and 60 g L-1, plus a zero sucrose medium) and macronutrient salts (complete Murashige and Skoog (MS) medium and half MS medium (with half-strength macronutrients)) on survival and development of the plantlets was evaluated. In all treatments 100% plantlet survival was achieved. The integrated analysis of height of aerial part, number of leaves per plantlet, fresh mass, number of roots per plantlet and length of the longest root showed that the plantlets exhibited...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Atlantic Forest; Carbohydrate; Conservation; In vitro culture; Orchids; Propagation.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000300292
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In vitro propagation of Anathallis adenochila (Loefgr.) F. Barros (Orchidaceae), a species endemic to southern and southeastern Brazil Acta Botanica
Endres Júnior,Delio; Sasamori,Márcio Hisayuki; Droste,Annette.
Anathallis adenochila is a small epiphytic orchid, endemic to the Atlantic Forest in the southern and southeastern regions of Brazil. Information on the species is scarce and limited to distribution and occurrence data, with no reports about its development. In the present study, plantlets were propagated in vitro, and the influence of different concentrations of macronutrient salts and sucrose on the survival and development of the species was assessed. The analysis were performed on complete Murashige and Skoog (MS) medium and half MS medium (with half-strength macronutrients) containing 10, 30 or 60 g L-1 of sucrose. Plantlet survival, height of the aerial part, and the length of the longest root were significantly greater on half MS medium containing...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conservation; Carbon source; Micropropagation; Orchid; Mineral salts.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000400001
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Survival and development of reintroduced Cattleya intermedia plants related to abiotic factors and herbivory at the edge and in the interior of a forest fragment in South Brazil Acta Botanica
Endres Júnior,Delio; Sasamori,Márcio Hisayuki; Schmitt,Jairo Lizandro; Droste,Annette.
ABSTRACT Biotic and abiotic factors, such as luminosity, temperature, air humidity, and herbivory, can affect the establishment of reintroduced plants in natural habitats. This study evaluated the effects of these factors on the survival and growth of Cattleya intermedia plants reintroduced into a forest fragment in South Brazil. Plants of C. intermedia were obtained from in vitro seed germination in asymbiotic culture. Eighty-eight plants were reintroduced at both the forest edge and forest interior. Plants with greater shoot heights and number of leaves and pseudobulbs suffered more damage from herbivores at the edge. There were no significant differences in morphometric parameters between damaged and non-damaged plants in the interior. Tenthecoris...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conservation; Micropropagation; Monitoring; Orchidaceae; Reintroduction.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000400555
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